5WFE
Cas1-Cas2-IHF-DNA holo-complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | CRISPR-associated endonuclease Cas1 | polymer | 305 | 33235.4 | 4 | UniProt (Q46896) Pfam (PF01867) In PDB | Escherichia coli K-12 | |
2 | E, F | CRISPR-associated endoribonuclease Cas2 | polymer | 103 | 11553.3 | 2 | UniProt (P45956) Pfam (PF09707) In PDB | Escherichia coli K-12 | |
3 | G | DNA (28-MER) | polymer | 28 | 8680.6 | 1 | synthetic construct | ||
4 | H | DNA (45-MER) | polymer | 61 | 18746.0 | 1 | synthetic construct | ||
5 | I | DNA (76-MER) | polymer | 95 | 29101.7 | 1 | synthetic construct | ||
6 | J | DNA (61-MER) | polymer | 62 | 19286.4 | 1 | synthetic construct | ||
7 | K | Integration host factor subunit alpha | polymer | 99 | 11374.0 | 1 | UniProt (B7MAS3) Pfam (PF00216) UniProt (by SIFTS) (P0A6X7) In PDB | Escherichia coli S88 | IHF-alpha |
8 | L | Integration host factor subunit beta | polymer | 94 | 10671.2 | 1 | UniProt (B7MHM1) Pfam (PF00216) UniProt (by SIFTS) (P0A6Y1) In PDB | Escherichia coli S88 | IHF-beta |
Sequence modifications
E, F: 1 - 94 (UniProt: P45956)
PDB | External Database | Details |
---|---|---|
Gly 95 | - | expression tag |
Ser 96 | - | expression tag |
Ser 97 | - | expression tag |
Glu 98 | - | expression tag |
Asn 99 | - | expression tag |
Leu 100 | - | expression tag |
Tyr 101 | - | expression tag |
Phe 102 | - | expression tag |
Gln 103 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 12 |
Total formula weight | 253908.1 | |
All* | Total formula weight | 253908.1 |