5WFE
Cas1-Cas2-IHF-DNA holo-complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | CRISPR-associated endonuclease Cas1 | polymer | 305 | 33235.4 | 4 | UniProt (Q46896) Pfam (PF01867) | Escherichia coli K-12 | |
| 2 | E, F (E, F) | CRISPR-associated endoribonuclease Cas2 | polymer | 103 | 11553.3 | 2 | UniProt (P45956) Pfam (PF09707) | Escherichia coli K-12 | |
| 3 | G (G) | DNA (28-MER) | polymer | 28 | 8680.6 | 1 | synthetic construct | ||
| 4 | H (H) | DNA (45-MER) | polymer | 61 | 18746.0 | 1 | synthetic construct | ||
| 5 | I (I) | DNA (76-MER) | polymer | 95 | 29101.7 | 1 | synthetic construct | ||
| 6 | J (J) | DNA (61-MER) | polymer | 62 | 19286.4 | 1 | synthetic construct | ||
| 7 | K (K) | Integration host factor subunit alpha | polymer | 99 | 11374.0 | 1 | UniProt (B7MAS3) Pfam (PF00216) UniProt (by SIFTS) (P0A6X7) | Escherichia coli S88 | IHF-alpha |
| 8 | L (L) | Integration host factor subunit beta | polymer | 94 | 10671.2 | 1 | UniProt (B7MHM1) Pfam (PF00216) UniProt (by SIFTS) (P0A6Y1) | Escherichia coli S88 | IHF-beta |
Sequence modifications
E, F: 1 - 94 (UniProt: P45956)
| PDB | External Database | Details |
|---|---|---|
| Gly 95 | - | expression tag |
| Ser 96 | - | expression tag |
| Ser 97 | - | expression tag |
| Glu 98 | - | expression tag |
| Asn 99 | - | expression tag |
| Leu 100 | - | expression tag |
| Tyr 101 | - | expression tag |
| Phe 102 | - | expression tag |
| Gln 103 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 253908.1 | |
| All* | Total formula weight | 253908.1 |
