5WFC
Humanized mutant of the Chaetomium thermophilum Polycomb Repressive Complex 2 bound to the inhibitor GSK343
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Polycomb Protein EED | polymer | 605 | 66021.4 | 1 | UniProt (G0S8H7) Pfam (PF00400) Pfam (PF22038) | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) | |
| 2 | B (B) | Histone-lysine-N-methyltransferase EZH2, Polycomb protein SUZ12 chimera | polymer | 936 | 106801.7 | 1 | UniProt (G0SDW4) UniProt (G0RYC6) Pfam (PF18601) Pfam (PF18600) Pfam (PF21509) Pfam (PF00856) Pfam (PF09733) | Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) | |
| 3 | C (D) | Histone H3.1 | polymer | 11 | 1146.4 | 1 | UniProt (P68431) | Homo sapiens | Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l |
| 4 | D, E, F, G, H... (B) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 5 | L (B) | N-[(6-methyl-2-oxo-4-propyl-1,2-dihydropyridin-3-yl)methyl]-6-[2-(4-methylpiperazin-1-yl)pyridin-4-yl]-1-(propan-2-yl)-1H-indazole-4-carboxamide | non-polymer | 541.7 | 1 | Chemie (A97) | |||
| 6 | M, N, O (A, B, D) | water | water | 18.0 | 636 | Chemie (HOH) |
Sequence modifications
A: 1 - 565 (UniProt: G0S8H7)
B: 191 - 2523 (UniProt: G0SDW4)
B: 2530 - 2691 (UniProt: G0RYC6)
| PDB | External Database | Details |
|---|---|---|
| Met -39 | - | initiating methionine |
| Ala -38 | - | expression tag |
| Ser -37 | - | expression tag |
| Ala -36 | - | expression tag |
| Trp -35 | - | expression tag |
| Ser -34 | - | expression tag |
| His -33 | - | expression tag |
| Pro -32 | - | expression tag |
| Gln -31 | - | expression tag |
| Phe -30 | - | expression tag |
| Glu -29 | - | expression tag |
| Lys -28 | - | expression tag |
| Gly -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Gly -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ala -16 | - | expression tag |
| Trp -15 | - | expression tag |
| Ser -14 | - | expression tag |
| His -13 | - | expression tag |
| Pro -12 | - | expression tag |
| Gln -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Glu -9 | - | expression tag |
| Lys -8 | - | expression tag |
| Leu -7 | - | expression tag |
| Glu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Leu -4 | - | expression tag |
| Phe -3 | - | expression tag |
| Gln -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Pro 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 182 | - | expression tag |
| Asn 183 | - | expression tag |
| His 184 | - | expression tag |
| His 185 | - | expression tag |
| His 186 | - | expression tag |
| His 187 | - | expression tag |
| His 188 | - | expression tag |
| His 189 | - | expression tag |
| Ala 190 | - | expression tag |
| Ile 302 | Pro 302 | engineered mutation |
| Tyr 304 | Arg 304 | engineered mutation |
| - | Glu 849 | deletion |
| Lys 850 | Glu 850 | engineered mutation |
| Tyr 851 | Asn 851 | engineered mutation |
| Met 852 | Lys 852 | engineered mutation |
| Cys 853 | Val 853 | engineered mutation |
| Phe 855 | Tyr 855 | engineered mutation |
| Leu 2524 | - | linker |
| Val 2525 | - | linker |
| Pro 2526 | - | linker |
| Arg 2527 | - | linker |
| Gly 2528 | - | linker |
| Ser 2529 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 173969.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1065.0 | |
| All* | Total formula weight | 175034.4 |
