5WDG

Acetolactate Synthase from Klebsiella pneumoniae in Complex with a Reaction Intermediate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Acetolactate synthase, catabolic	polymer	579	62555.1	2	UniProt (P27696) Pfam (PF02776) Pfam (PF00205) Pfam (PF02775)	Klebsiella pneumoniae	ALS
2	C, D, E, I, J... (A, B)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
3	F, L (A, B)	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
4	G, M (A, B)	(2S,3S)-2,3-dihydroxy-3-[(7S,8R,9aS)-8-(2-{[(R)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-2,7-dimethyl-5,7,8,10-tetrahydro-9aH-pyrimido[4,5-d][1,3]thiazolo[3,2-a]pyrimidin-9a-yl]-2-methylbutanoic acid	non-polymer		558.4	2	Chemie (A4Y)
5	H, N (A, B)	PYRUVIC ACID	non-polymer		88.1	2	Chemie (PYR)
6	O, P (A, B)	water	water		18.0	496	Chemie (HOH)

Sequence modifications

A, B: 1 - 559 (UniProt: P27696)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 407	Thr 407	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	125110.2
Non-Polymers*	Number of molecules	12
	Total formula weight	1628.8
All*	Total formula weight	126739.0

*Water molecules are not included.