5WB2

US28 bound to engineered chemokine CX3CL1.35 and nanobodies

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Envelope protein US28, nanobody 7 fusion protein	polymer	460	52158.4	1	UniProt (Q80KM9) Pfam (PF00001) UniProt (by SIFTS) (P69333) In PDB	Human cytomegalovirus (HHV-5)	US28 protein
2	B	CX3CL1 protein	polymer	76	8631.1	1	UniProt (Q6I9S9) Pfam (PF00048) UniProt (by SIFTS) (P78423) In PDB	Homo sapiens (Human)	engineered chemokine CX3CL1.35
3	D	nanobody B1	polymer	131	14151.6	1		synthetic construct
4	A	CHOLESTEROL	non-polymer		386.7	2	Chemie (CLR)
5	A	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	4	Chemie (OLC)
6	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
7	B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)

Sequence modifications

A: 1 - 982 (UniProt: Q80KM9)

PDB	External Database	Details
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Ala 0	-	expression tag

A: 983 - 1125 (PDB: 5WB2)
B: 10 - 76 (UniProt: Q6I9S9)

PDB	External Database	Details
Leu 1	-	expression tag
Leu 2	-	expression tag
Pro 3	-	expression tag
His 4	-	expression tag
Ala 5	-	expression tag
Asn 6	-	expression tag
Tyr 7	-	expression tag
Cys 8	-	expression tag
Ala 9	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	74941.1
Non-Polymers*	Number of molecules	8
	Total formula weight	2460.1
All*	Total formula weight	77401.2

*Water molecules are not included.