5W7C

Human acyloxyacyl hydrolase (AOAH), proteolytically processed, S263A mutant, with LPS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Acyloxyacyl hydrolase	polymer	139	15831.4	2	UniProt (P28039) Pfam (PF20825)	Homo sapiens (Human)
2	B, D (C, D)	Acyloxyacyl hydrolase	polymer	423	48170.4	2	UniProt (P28039) Pfam (PF00657)	Homo sapiens (Human)
3	E, F, G, H (E, F, G, H)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	4	In PDB GlyTouCan (G42666HT)
4	I, N, R, W (A, C, B, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
5	J, P, Q, S, Y... (A, C, B, D)	3-HYDROXY-TETRADECANOIC ACID	non-polymer		244.4	6	Chemie (FTT)
6	K, L, M, T, U... (C, D)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
7	O, X (C, D)	LAURIC ACID	non-polymer		200.3	2	Chemie (DAO)
8	AA, BA, CA, DA (A, C, B, D)	water	water		18.0	295	Chemie (HOH)

Sequence modifications

A, B: 24 - 152 (UniProt: P28039)

PDB	External Database	Details
Asp 14	-	expression tag
Arg 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
His 21	-	expression tag
Lys 22	-	expression tag
Leu 23	-	expression tag

C, D: 153 - 575 (UniProt: P28039)

PDB	External Database	Details
Ala 263	Ser 263	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128003.6
Branched	Number of molecules	4
	Total formula weight	1697.6
Non-Polymers*	Number of molecules	18
	Total formula weight	2992.2
All*	Total formula weight	132693.4

*Water molecules are not included.