5W7B

Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, B)	Acyloxyacyl hydrolase small subunit	polymer	141	16385.0	2	UniProt (O18823) Pfam (PF03489)	Oryctolagus cuniculus (Rabbit)
2	B, D (C, D)	Acyloxyacyl hydrolase large subunit	polymer	422	47621.8	2	UniProt (O18823) Pfam (PF00657)	Oryctolagus cuniculus (Rabbit)
3	E, I (E, I)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	2	In PDB GlyTouCan (G86851RC)
4	F, J (F, J)	3-deoxy-alpha-D-manno-oct-2-ulopyranosonic acid-(2-6)-2-amino-2-deoxy-beta-D-glucopyranose-(1-6)-2-amino-2-deoxy-alpha-D-glucopyranose	branched		560.5	2	In PDB GlyTouCan (G74817EY)
5	G, K (G, K)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	2	In PDB GlyTouCan (G21290RB)
6	H, L (H, L)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
7	AA, AB, BA, BB, CA... (C, D, A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	16	Chemie (PEG)
8	CB, DB, EB, GA, HA... (D, C, A)	TRIETHYLENE GLYCOL	non-polymer		150.2	7	Chemie (PGE)
9	JB, KB, NA, OA, P... (D, C, A, B)	3-HYDROXY-TETRADECANOIC ACID	non-polymer		244.4	6	Chemie (FTT)
10	LB, PA, Q, SA (D, C, A, B)	MYRISTIC ACID	non-polymer		228.4	4	Chemie (MYR)
11	R, S, T, TA, UA... (C, D)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
12	U, WA (C, D)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
13	QA, V, XA (B, C, D)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	3	Chemie (MES)
14	FB, GB, JA, KA (D, C)	PENTAETHYLENE GLYCOL	non-polymer		238.3	4	Chemie (1PE)
15	HB, IB, LA, MA (D, C)	PHOSPHATE ION	non-polymer		95.0	4	Chemie (PO4)
16	MB, NB, OB, PB (A, C, B, D)	water	water		18.0	448	Chemie (HOH)

Sequence modifications

A, B: 23 - 153 (UniProt: O18823)

PDB	External Database	Details
Asp 13	-	expression tag
Arg 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Lys 21	-	expression tag
Leu 22	-	expression tag

C, D: 154 - 575 (UniProt: O18823)

PDB	External Database	Details
Ala 262	Ser 262	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	128013.5
Branched	Number of molecules	8
	Total formula weight	3845.6
Non-Polymers*	Number of molecules	52
	Total formula weight	7480.1
All*	Total formula weight	139339.2

*Water molecules are not included.