5W7A

Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS (low quality saposin domain)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Acyloxyacyl hydrolase small subunit	polymer	141	16385.0	1	UniProt (O18823) Pfam (PF03489) In PDB	Oryctolagus cuniculus (Rabbit)
2	B	Acyloxyacyl hydrolase large subunit	polymer	422	47621.8	1	UniProt (O18823) Pfam (PF00657) In PDB	Oryctolagus cuniculus (Rabbit)
3	C	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	B	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
5	B	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
7	B	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
8	B	3-HYDROXY-TETRADECANOIC ACID	non-polymer		244.4	1	Chemie (FTT)
9		water	water		18.0	111	Chemie (HOH)

Sequence modifications

A: 23 - 153 (UniProt: O18823)

PDB	External Database	Details
Asp 13	-	expression tag
Arg 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Lys 21	-	expression tag
Leu 22	-	expression tag

B: 154 - 575 (UniProt: O18823)

PDB	External Database	Details
Ala 262	Ser 262	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64006.8
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	7
	Total formula weight	963.1
All*	Total formula weight	65394.3

*Water molecules are not included.