5W7A
Rabbit acyloxyacyl hydrolase (AOAH), proteolytically processed, S262A mutant, with LPS (low quality saposin domain)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-23 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97243 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.578, 123.199, 125.918 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.686 - 2.300 |
R-factor | 0.2412 |
Rwork | 0.240 |
R-free | 0.26150 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.746 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 31484 |
<I/σ(I)> | 45 |
Completeness [%] | 100.0 |
Redundancy | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | post-trypsin; 1 mM Triton X-100, 0.333 mM E. coli LPS Ra; 1 M sodium citrate pH 7 |