5W5C

Crystal structure of the primed SNARE-Complexin-Synaptotagmin-1 C2AB complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Vesicle-associated membrane protein 2	polymer	40	4704.2	1	UniProt (P63045) Pfam (PF00957)	Rattus norvegicus (Rat)	VAMP-2,Synaptobrevin-2
2	B (B)	Syntaxin-1A	polymer	67	7838.0	1	UniProt (P32851) Pfam (PF05739)	Rattus norvegicus (Rat)	Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A
3	C (C)	Synaptosomal-associated protein 25	polymer	77	9030.1	1	UniProt (P60881)	Rattus norvegicus (Rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
4	D (D)	Synaptosomal-associated protein 25	polymer	65	7471.4	1	UniProt (P60881)	Rattus norvegicus (Rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
5	E (E)	Complexin-1	polymer	83	9612.9	1	UniProt (P63041) Pfam (PF05835)	Rattus norvegicus (Rat)	Complexin I,CPX I,Synaphin-2
6	F (F)	Synaptotagmin-1	polymer	282	32376.3	1	UniProt (P21707) Pfam (PF00168)	Rattus norvegicus (Rat)	Synaptotagmin I,SytI,p65
7	G (C)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
8	H, I, J, K, L (D, F)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
9	M, N, O, P, Q... (A, B, C, D, E...)	water	water		18.0	279	Chemie (HOH)

Sequence modifications

A: 28 - 66 (UniProt: P63045)

PDB	External Database	Details
Gly 27	-	expression tag

B: 191 - 256 (UniProt: P32851)

PDB	External Database	Details
Met 190	-	initiating methionine

D: 141 - 204 (UniProt: P60881)

PDB	External Database	Details
Met 140	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	71032.9
Non-Polymers*	Number of molecules	6
	Total formula weight	484.8
All*	Total formula weight	71517.7

*Water molecules are not included.