5W4L
Crystal structure of the non-neutralizing and ADCC-potent C11-like antibody N12-i3 in complex with HIV-1 clade A/E gp120, the CD4 mimetic M48U1, and the antibody N5-i5.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | G, A | clade A/E 93TH057 HIV-1 gp120 core | polymer | 385 | 42847.7 | 2 | UniProt (A0A0M3KKW9) Pfam (PF00516) In PDB | Human immunodeficiency virus 1 | |
| 2 | N, M | CD4 mimetic peptide M48U1 | polymer | 28 | 3056.8 | 2 | BIRD (PRD_001094) | synthetic construct | |
| 3 | H, F | Antibody N5-i5 Fab heavy chain | polymer | 226 | 23789.6 | 2 | Homo sapiens | ||
| 4 | L, I | Antibody N5-i5 light chain | polymer | 217 | 22943.2 | 2 | Homo sapiens | ||
| 5 | D, B | Antibody N12-i3 Fab heavy chain | polymer | 225 | 24170.0 | 2 | Homo sapiens | ||
| 6 | E, C | Antibody N12-i3 light chain | polymer | 215 | 23485.1 | 2 | Homo sapiens | ||
| 7 | A, G | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 21 | Chemie (NAG) | |||
| 8 | A, N | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 9 | water | water | 18.0 | 20 | Chemie (HOH) |
Sequence modifications
G, A: 44 - 492 (UniProt: A0A0M3KKW9)
| PDB | External Database | Details |
|---|---|---|
| Ser 31 | - | see sequence details |
| Asp 32 | - | see sequence details |
| Asn 33 | - | see sequence details |
| Leu 34 | - | see sequence details |
| Trp 35 | - | see sequence details |
| Val 36 | - | see sequence details |
| Thr 37 | - | see sequence details |
| Val 38 | - | see sequence details |
| Tyr 39 | - | see sequence details |
| Tyr 40 | - | see sequence details |
| Gly 41 | - | see sequence details |
| Val 42 | - | see sequence details |
| Pro 43 | - | see sequence details |
| Ser 375 | His 242 | engineered mutation |
| Pro 493 | - | see sequence details |
| Leu 494 | - | see sequence details |
| Gly 495 | - | see sequence details |
| Ile 496 | - | see sequence details |
| Ala 497 | - | see sequence details |
| Pro 498 | - | see sequence details |
| Thr 499 | - | see sequence details |
| Lys 500 | - | see sequence details |
| Ala 501 | - | see sequence details |
| Lys 502 | - | see sequence details |
| Arg 503 | - | see sequence details |
| Arg 504 | - | see sequence details |
| Val 505 | - | see sequence details |
| Val 506 | - | see sequence details |
| Gln 507 | - | see sequence details |
| Arg 508 | - | see sequence details |
| Glu 509 | - | see sequence details |
| Lys 510 | - | see sequence details |
| Arg 511 | - | see sequence details |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 280584.9 | |
| Non-Polymers* | Number of molecules | 23 |
| Total formula weight | 4716.3 | |
| All* | Total formula weight | 285301.2 |
