5W3J
Yeast microtubule stabilized with Taxol assembled from mutated tubulin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Tubulin alpha-1 chain | polymer | 447 | 49853.9 | 1 | UniProt (P09733) Pfam (PF00091) Pfam (PF03953) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | |
| 2 | B | Tubulin beta chain | polymer | 457 | 51089.7 | 1 | UniProt (P02557) Pfam (PF00091) Pfam (PF03953) In PDB | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Beta-tubulin |
| 3 | A | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 4 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 5 | B | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 6 | B | TAXOL | non-polymer | 853.9 | 1 | Chemie (TA1) |
Sequence modifications
B: 1 - 457 (UniProt: P02557)
| PDB | External Database | Details |
|---|---|---|
| Lys 19 | Ala 19 | engineered mutation |
| Val 23 | Thr 23 | engineered mutation |
| Asp 26 | Gly 26 | engineered mutation |
| His 227 | Asn 227 | engineered mutation |
| Phe 270 | Tyr 270 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 100943.5 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1844.6 | |
| All* | Total formula weight | 102788.1 |
