5W25

Crystal structure of Aspartyl-tRNA synthetase from Mycobacterium tuberculosis complexed with L-Aspartic Acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Aspartate--tRNA(Asp/Asn) ligase	polymer	616	67441.0	2	UniProt (P9WFW3) Pfam (PF01336) Pfam (PF00152) Pfam (PF02938)	Mycobacterium tuberculosis	Aspartyl-tRNA synthetase,AspRS,Non-discriminating aspartyl-tRNA synthetase,ND-AspRS
2	C, G (A, B)	ASPARTIC ACID	non-polymer		133.1	2	Chemie (ASP)		Aspartyl-tRNA synthetase,AspRS,Non-discriminating aspartyl-tRNA synthetase,ND-AspRS
3	D, E, H (A, B)	LYSINE	non-polymer		147.2	3	Chemie (LYS)
4	F, I (A, B)	GLUTAMIC ACID	non-polymer		147.1	2	Chemie (GLU)
5	J, K (A, B)	water	water		18.0	42	Chemie (HOH)

Sequence modifications

A, B: 2 - 609 (UniProt: P9WFW3)

PDB	External Database	Details
Met -21	-	initiating methionine
Gly -20	-	expression tag
Ser -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
Gly -9	-	expression tag
Leu -8	-	expression tag
Val -7	-	expression tag
Pro -6	-	expression tag
Arg -5	-	expression tag
Gly -4	-	expression tag
Ser -3	-	expression tag
His -2	-	expression tag
Val -1	-	expression tag
Phe 0	-	expression tag
Val 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	134882.0
Non-Polymers*	Number of molecules	7
	Total formula weight	1002.0
All*	Total formula weight	135884.0

*Water molecules are not included.