5W21

Crystal Structure of a 1:1:1 FGF23-FGFR1c-aKlotho Ternary Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Klotho	polymer	981	112666.1	1	UniProt (Q9UEF7) Pfam (PF00232)	Homo sapiens (Human)	alpha-Klotho
2	B (B)	Fibroblast growth factor 23	polymer	226	25406.5	1	UniProt (Q9GZV9) Pfam (PF00167)	Homo sapiens (Human)	FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor
3	C (C)	Fibroblast growth factor receptor 1	polymer	226	25376.0	1	UniProt (P11362) Pfam (PF07679)	Homo sapiens (Human)	FGFR-1, Basic fibroblast growth factor receptor 1, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, N-sam, Proto-oncogene c-Fgr
4	D, E, F, G, H... (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	7	Chemie (NAG)
5	K (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	L (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

B: 25 - 204 (UniProt: Q9GZV9)

PDB	External Database	Details
Met -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Ser -14	-	expression tag
Ser -13	-	expression tag
Gly -12	-	expression tag
Leu -11	-	expression tag
Val -10	-	expression tag
Pro -9	-	expression tag
Arg -8	-	expression tag
Gly -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
Met -4	-	expression tag
Lys -3	-	expression tag
Glu -2	-	expression tag
Thr -1	-	expression tag
Ala 0	-	expression tag
Ala 1	-	expression tag
Ala 2	-	expression tag
Lys 3	-	expression tag
Phe 4	-	expression tag
Glu 5	-	expression tag
Arg 6	-	expression tag
Gln 7	-	expression tag
His 8	-	expression tag
Met 9	-	expression tag
Asp 10	-	expression tag
Ser 11	-	expression tag
Pro 12	-	expression tag
Asp 13	-	expression tag
Leu 14	-	expression tag
Gly 15	-	expression tag
Thr 16	-	expression tag
Asp 17	-	expression tag
Asp 18	-	expression tag
Asp 19	-	expression tag
Asp 20	-	expression tag
Lys 21	-	expression tag
Ala 22	-	expression tag
Met 23	-	expression tag
Gly 24	-	expression tag
Ala 140	Arg 140	conflict
Ala 143	Arg 143	conflict
Gln 176	Arg 176	conflict
Gln 179	Arg 179	conflict

C: 142 - 365 (UniProt: P11362)

PDB	External Database	Details
Met 140	-	expression tag
Ala 141	-	expression tag
Gln 185	Asn 185	conflict

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	163448.6
Non-Polymers*	Number of molecules	8
	Total formula weight	1613.9
All*	Total formula weight	165062.4

*Water molecules are not included.