5W21

Crystal Structure of a 1:1:1 FGF23-FGFR1c-aKlotho Ternary Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	283.301, 72.593, 95.329
Unit cell angles	90.00, 91.98, 90.00

Refinement procedure

Resolution	48.801 - 3.000
R-factor	0.2324
Rwork	0.230
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 2P39 1cvs & 2DGA
RMSD bond length	0.002
RMSD bond angle	0.483
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000	3.000
Number of reflections	58000
<I/σ(I)>	11.1
Completeness [%]	99.7
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	22% PEG350 MME, 0.1 M Tris-HCl, pH 8.0, 1 mM reduced glutathione, 1 mM oxidized glutathione