5W1T

X-ray crystal structure of Escherichia coli RNA polymerase and DksA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	4	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	2	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	2	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, K	RpoZ	polymer	91	10249.5	2	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)
5	F, L	RNA polymerase sigma factor RpoD	polymer	613	70352.2	2	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli (strain K12)	Sigma-70
6	M, N	RNA polymerase-binding transcription factor DksA	polymer	151	17554.8	2	UniProt (P0ABS1) Pfam (PF21157) Pfam (PF01258) In PDB	Escherichia coli (strain K12)	DnaK suppressor protein
7	D, J	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	D, J, M, N	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	954923.2
Non-Polymers*	Number of molecules	8
	Total formula weight	441.1
All*	Total formula weight	955364.2

*Water molecules are not included.