5W1T

X-ray crystal structure of Escherichia coli RNA polymerase and DksA complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	184.000, 204.894, 314.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.081 - 4.500
R-factor	0.2212
Rwork	0.220
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yg2
RMSD bond length	0.007
RMSD bond angle	1.147
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.580
High resolution limit [Å]	4.500	4.500
Rpim	0.046	0.776
Number of reflections	70723	3518
<I/σ(I)>	16.6
Completeness [%]	99.9	99.9
Redundancy	6.7	5.7
CC(1/2)		0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	0.1M HEPES-HCL (PH7), 0.2M CA-ACETATE, 15% PEG400