5W1S

X-ray crystal structure of Escherichia coli RNA polymerase and TraR complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	4	UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) In PDB	Escherichia coli (strain K12)	RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha
2	C, I	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	2	UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) In PDB	Escherichia coli (strain K12)	RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta
3	D, J	DNA-directed RNA polymerase subunit beta'	polymer	1407	155366.8	2	UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB	Escherichia coli (strain K12)	RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta'
4	E, K	DNA-directed RNA polymerase subunit omega	polymer	91	10249.5	2	UniProt (P0A800) Pfam (PF01192) In PDB	Escherichia coli (strain K12)	RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega
5	F, L	RNA polymerase sigma factor RpoD	polymer	613	70352.2	2	UniProt (P00579) Pfam (PF03979) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB	Escherichia coli (strain K12)	Sigma-70
6	M, N	Protein TraR	polymer	79	9153.4	2	UniProt (P41065) Pfam (PF01258) In PDB	Escherichia coli (strain K12)
7	D, J	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
8	D, J, M, N	ZINC ION	non-polymer		65.4	6	Chemie (ZN)

Sequence modifications

M, N: 1 - 73 (UniProt: P41065)

PDB	External Database	Details
His 74	-	expression tag
His 75	-	expression tag
His 76	-	expression tag
His 77	-	expression tag
His 78	-	expression tag
His 79	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	938120.4
Non-Polymers*	Number of molecules	8
	Total formula weight	441.1
All*	Total formula weight	938561.4

*Water molecules are not included.