5W0Z

Crystal structure of MBP fused activation-induced cytidine deaminase (AID)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	B, A	MBP fused activation-induced cytidine deaminase	polymer	549	61717.9	2	UniProt (P0AEY0) UniProt (Q9GZX7) Pfam (PF01547) Pfam (PF08210) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli O157:H7
2	B, A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

B, A: 2 - 367 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	initiating methionine
Ala 83	Asp 108	engineered mutation
Ala 84	Lys 109	engineered mutation
Ala 173	Glu 198	engineered mutation
Ala 174	Asn 199	engineered mutation
Ala 240	Lys 265	engineered mutation
Ala 360	Glu 385	engineered mutation
Ala 363	Lys 388	engineered mutation
Ala 364	Asp 389	engineered mutation
Asn 368	-	linker
Ala 369	-	linker
Ala 370	-	linker
Ala 371	-	linker
Glu 372	-	linker
Phe 373	-	linker
Met 1006	-	linker
Asp 1007	-	linker
Pro 1008	-	linker
Ala 1009	-	linker
Thr 1010	-	linker
Phe 1011	-	linker
Thr 1012	-	linker

B, A: 1013 - 1181 (UniProt: Q9GZX7)

PDB	External Database	Details
Glu 1042	Phe 42	engineered mutation
Ala 1130	His 130	engineered mutation
Glu 1131	Arg 131	engineered mutation
Tyr 1141	Phe 141	engineered mutation
Glu 1145	Phe 145	engineered mutation
Gln 1181	Leu 181	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	123435.8
Non-Polymers*	Number of molecules	2
	Total formula weight	130.8
All*	Total formula weight	123566.6

*Water molecules are not included.