5W0Z
Crystal structure of MBP fused activation-induced cytidine deaminase (AID)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.680, 167.650, 188.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.610 |
| R-factor | 0.27601 |
| Rwork | 0.274 |
| R-free | 0.30693 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5w0r |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 188.300 | 3.740 |
| High resolution limit [Å] | 3.600 | 3.610 |
| Rpim | 0.743 | |
| Number of reflections | 12574 | 11572 |
| <I/σ(I)> | 6.4 | 1 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.9 | 8.1 |
| CC(1/2) | 0.688 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.26 M NaCl, 0.1 M MES pH 6.0, 12% PEG3350 |
