5W0Z

Crystal structure of MBP fused activation-induced cytidine deaminase (AID)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-03-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	38.680, 167.650, 188.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 3.610
R-factor	0.27601
Rwork	0.274
R-free	0.30693
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5w0r
RMSD bond length	0.008
RMSD bond angle	1.201
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	188.300	3.740
High resolution limit [Å]	3.600	3.610
Rpim		0.743
Number of reflections	12574	11572
<I/σ(I)>	6.4	1
Completeness [%]	99.8	99.8
Redundancy	7.9	8.1
CC(1/2)		0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	0.26 M NaCl, 0.1 M MES pH 6.0, 12% PEG3350