5VYT

Crystal structure of the WbkC N-formyltransferase (F142A variant) from Brucella melitensis

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase	polymer	261	29235.6	4	UniProt (F8WJP6) Pfam (PF00551) In PDB	Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
2	A, B, C, D	N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid	non-polymer		473.4	4	Chemie (FON)
3	A, B, C, D	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	4	Chemie (GDP)
4	C	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5		water	water		18.0	306	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 259 (UniProt: F8WJP6)

PDB	External Database	Details
Gly -1	-	expression tag
His 0	-	expression tag
Ala 78	Asp 78	engineered mutation
Ala 142	Phe 142	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	116942.2
Non-Polymers*	Number of molecules	10
	Total formula weight	3737.5
All*	Total formula weight	120679.7

*Water molecules are not included.