5VYT
Crystal structure of the WbkC N-formyltransferase (F142A variant) from Brucella melitensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase | polymer | 261 | 29235.6 | 4 | UniProt (F8WJP6) | Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) | |
| 2 | E, G, I, M (A, B, C, D) | N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid | non-polymer | 473.4 | 4 | Chemie (FON) | |||
| 3 | F, H, J, N (A, B, C, D) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 4 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 4 | K, L (C) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) PubChem (312) | |||
| 5 | O, P, Q, R (A, B, C, D) | water | water | 18.0 | 306 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 259 (UniProt: F8WJP6)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 78 | Asp 78 | engineered mutation |
| Ala 142 | Phe 142 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 116942.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3737.5 | |
| All* | Total formula weight | 120679.7 |






