5VYT
Crystal structure of the WbkC N-formyltransferase (F142A variant) from Brucella melitensis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Gdp-mannose 4,6-dehydratase / gdp-4-amino-4,6-dideoxy-d-mannose formyltransferase | polymer | 261 | 29235.6 | 4 | UniProt (F8WJP6) Pfam (PF00551) | Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) | |
| 2 | E, G, I, M (A, B, C, D) | N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid | non-polymer | 473.4 | 4 | Chemie (FON) | |||
| 3 | F, H, J, N (A, B, C, D) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 4 | Chemie (GDP) | |||
| 4 | K, L (C) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 5 | O, P, Q, R (A, B, C, D) | water | water | 18.0 | 306 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 259 (UniProt: F8WJP6)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 78 | Asp 78 | engineered mutation |
| Ala 142 | Phe 142 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 116942.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 3737.5 | |
| All* | Total formula weight | 120679.7 |
