5VYK

Crystal structure of the BRS domain of BRAF in complex with the CC-SAM domain of KSR1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Chimera protein of BRS domain of BRAF and CC-SAM domain of KSR1,Serine/threonine-protein kinase B-raf	polymer	232	25583.9	2	UniProt (Q8IVT5) UniProt (P15056) Pfam (PF20406)	Homo sapiens (Human)	Proto-oncogene B-Raf,p94,v-Raf murine sarcoma viral oncogene homolog B1
2	C, D (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
3	E, F (A, C)	water	water		18.0	99	Chemie (HOH)

Sequence modifications

A, C: 27 - 172 (UniProt: Q8IVT5)

PDB	External Database	Details
Gly 24	-	expression tag
Ala 25	-	expression tag
Met 26	-	expression tag
Gly 173	-	linker
Ser 174	-	linker
Gly 175	-	linker
Ser 176	-	linker
Gly 177	-	linker
Ser 178	-	linker
Gly 179	-	linker
Ser 180	-	linker

A, C: 1036 - 1110 (UniProt: P15056)

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	51167.8
Non-Polymers*	Number of molecules	2
	Total formula weight	184.2
All*	Total formula weight	51352.0

*Water molecules are not included.