5VYK

Crystal structure of the BRS domain of BRAF in complex with the CC-SAM domain of KSR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	179.796, 54.336, 51.864
Unit cell angles	90.00, 106.68, 90.00

Refinement procedure

Resolution	49.641 - 1.749
R-factor	0.2185
Rwork	0.217
R-free	0.23920
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.750
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.749	1.749
Rmeas		2.810
Number of reflections	47556	6677
<I/σ(I)>	14.5
Completeness [%]	97.9
Redundancy	3.9
CC(1/2)		0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M Tris pH 7.5, 3.0M sodium formate