5VYF

Structure of single chain Fel d 1 bound to a neutralizing antibody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (L, A)	Antibody light chain	polymer	214	23400.0	2		Mus musculus
2	B, E (H, B)	Antibody Fab heavy chain	polymer	220	23473.2	2		Mus musculus
3	C, F (F, C)	Chimera of Major allergen I polypeptide chain 2,Major allergen I polypeptide chain 1	polymer	199	21919.9	2	UniProt (P30440) UniProt (P30438) Pfam (PF09252) Pfam (PF01099)	Felis catus (Cat)	AG4,Allergen Cat-1,Allergen Fel d I-B,Allergen FdI,AG4,Allergen Cat-1,Allergen Fel d I-A,Allergen Fel dI
4	G, H (F, C)	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	I, J, K, L, M... (L, H, F, A, B...)	water	water		18.0	101	Chemie (HOH)

Sequence modifications

F, C: 1 - 92 (UniProt: P30440)

PDB	External Database	Details
Ala 33	Asn 50	engineered mutation

F, C: 93 - 162 (UniProt: P30438)

PDB	External Database	Details
Leu 152	Val 82	variant
Gly 163	-	expression tag
Pro 164	-	expression tag
Gly 165	-	expression tag
Gly 166	-	expression tag
Glu 167	-	expression tag
Gln 168	-	expression tag
Lys 169	-	expression tag
Leu 170	-	expression tag
Ile 171	-	expression tag
Ser 172	-	expression tag
Glu 173	-	expression tag
Glu 174	-	expression tag
Asp 175	-	expression tag
Leu 176	-	expression tag
Gly 177	-	expression tag
Gly 178	-	expression tag
Glu 179	-	expression tag
Gln 180	-	expression tag
Lys 181	-	expression tag
Leu 182	-	expression tag
Ile 183	-	expression tag
Ser 184	-	expression tag
Glu 185	-	expression tag
Glu 186	-	expression tag
Asp 187	-	expression tag
Leu 188	-	expression tag
Ser 189	-	expression tag
Gly 190	-	expression tag
His 191	-	expression tag
His 192	-	expression tag
His 193	-	expression tag
His 194	-	expression tag
His 195	-	expression tag
His 196	-	expression tag
Ser 197	-	expression tag
Ser 198	-	expression tag
Gly 199	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	137586.2
Non-Polymers*	Number of molecules	2
	Total formula weight	80.2
All*	Total formula weight	137666.3

*Water molecules are not included.