5VU0

Crystal structure of the complex between afucosylated/galactosylated human IgG1 Fc and Fc gamma receptor IIIa (CD16A) with Man5 N-glycans

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Immunoglobulin gamma-1 heavy chain	polymer	217	24512.7	2	UniProt (P0DOX5) Pfam (PF07654)	Homo sapiens (Human)	Immunoglobulin gamma-1 heavy chain NIE
2	C (C)	Low affinity immunoglobulin gamma Fc region receptor III-A	polymer	172	19727.1	1	UniProt (H0Y755) Pfam (PF13895) UniProt (by SIFTS) (P08637)	Homo sapiens (Human)
3	D, E (D, E)	beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1479.3	2	In PDB GlyTouCan (G89993FE)
4	F, G, P, X, Y (A, B, C)	1,2-ETHANEDIOL	non-polymer		62.1	5	Chemie (EDO)
5	H, I, J, Q (A, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	4	Chemie (PEG)
6	K, L, M, N, O... (A, B)	SODIUM ION	non-polymer		23.0	9	Chemie (NA)
7	V, W (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)
8	AA, BA, Z (B, C, A)	water	water		18.0	254	Chemie (HOH)

Sequence modifications

C: 3 - 174 (UniProt: H0Y755)

PDB	External Database	Details
Gln 38	Asn 109	engineered mutation
Gln 74	Asn 145	engineered mutation
Val 158	Phe 229	engineered mutation
Gln 169	Asn 240	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	68752.5
Branched	Number of molecules	2
	Total formula weight	2958.7
Non-Polymers*	Number of molecules	20
	Total formula weight	1384.1
All*	Total formula weight	73095.4

*Water molecules are not included.