5VS0

Human DNA polymerase beta 8-oxoG:dC extension with dTTP after 80 s

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA polymerase beta	polymer	341	39070.5	1	UniProt (P06746) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)
2	D	DNA (5'-D(P*GP*TP*CP*GP*G)-3')	polymer	5	1536.0	1		synthetic construct
3	P	DNA (5'-D(*CP*TP*GP*AP*TP*GP*CP*GP*CP*CP*T)-3')	polymer	11	3325.2	1		synthetic construct
4	T	DNA (5'-D(*CP*CP*GP*AP*CP*AP*(8OG)P*GP*CP*GP*CP*AP*TP*CP*AP*G)-3')	polymer	16	4909.2	1		synthetic construct
5	A, P	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
6	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7	A	IMIDAZOLE	non-polymer		69.1	1	Chemie (IMD)
8	A	PYROPHOSPHATE	non-polymer		178.0	1	Chemie (PPV)
9		water	water		18.0	145	Chemie (HOH)

Sequence modifications

A: 1 - 335 (UniProt: P06746)

PDB	External Database	Details
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	48840.9
Non-Polymers*	Number of molecules	10
	Total formula weight	463.8
All*	Total formula weight	49304.7

*Water molecules are not included.