5VRH

Apolipoprotein N-acyltransferase C387S active site mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Apolipoprotein N-acyltransferase	polymer	522	58186.4	1	UniProt (P23930) Pfam (PF20154) Pfam (PF00795)	Escherichia coli (strain K12)	ALP N-acyltransferase,Copper homeostasis protein CutE
2	B, C (A)	(2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	2	Chemie (OLB)
3	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
4	E, F (A)	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
5	G (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	H, I (A)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	2	Chemie (OLC)
7	J (A)	water	water		18.0	258	Chemie (HOH)

Sequence modifications

A: 2 - 512 (UniProt: P23930)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 387	Cys 387	engineered mutation
Gly 513	-	expression tag
Asn 514	-	expression tag
Ser 515	-	expression tag
His 516	-	expression tag
His 517	-	expression tag
His 518	-	expression tag
His 519	-	expression tag
His 520	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	58186.4
Non-Polymers*	Number of molecules	8
	Total formula weight	1998.8
All*	Total formula weight	60185.2

*Water molecules are not included.