5VOH
Crystal structure of engineered water-forming NADPH oxidase (TPNOX) bound to NADPH. The G159A, D177A, A178R, M179S, P184R mutant of LbNOX.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | NADH oxidase | polymer | 518 | 56216.8 | 4 | UniProt (M5B0V2) Pfam (PF07992) Pfam (PF02852) | Lactobacillus brevis KB290 | |
| 2 | E, K, O, S (A, B, C, D) | FLAVIN-ADENINE DINUCLEOTIDE | non-polymer | 785.5 | 4 | Chemie (FAD) | |||
| 3 | F, L, P, T (A, B, C, D) | NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE | non-polymer | 745.4 | 4 | Chemie (NDP) | |||
| 4 | G, H, M, N, Q... (A, B, C, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 8 | Chemie (EDO) | |||
| 5 | I (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 6 | J, W (A, D) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 7 | AA, X, Y, Z (D, A, B, C) | water | water | 18.0 | 281 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 450 (UniProt: M5B0V2)
| PDB | External Database | Details |
|---|---|---|
| Met -49 | - | initiating methionine |
| His -48 | - | expression tag |
| His -47 | - | expression tag |
| His -46 | - | expression tag |
| His -45 | - | expression tag |
| His -44 | - | expression tag |
| His -43 | - | expression tag |
| Ser -42 | - | expression tag |
| Ser -41 | - | expression tag |
| Gly -40 | - | expression tag |
| Leu -39 | - | expression tag |
| Val -38 | - | expression tag |
| Pro -37 | - | expression tag |
| Arg -36 | - | expression tag |
| Gly -35 | - | expression tag |
| Ser -34 | - | expression tag |
| Gly -33 | - | expression tag |
| Met -32 | - | expression tag |
| Lys -31 | - | expression tag |
| Glu -30 | - | expression tag |
| Thr -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Ala -27 | - | expression tag |
| Ala -26 | - | expression tag |
| Lys -25 | - | expression tag |
| Phe -24 | - | expression tag |
| Glu -23 | - | expression tag |
| Arg -22 | - | expression tag |
| Gln -21 | - | expression tag |
| His -20 | - | expression tag |
| Met -19 | - | expression tag |
| Asp -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Asp -15 | - | expression tag |
| Leu -14 | - | expression tag |
| Gly -13 | - | expression tag |
| Thr -12 | - | expression tag |
| Asp -11 | - | expression tag |
| Asp -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Asp -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Ala -6 | - | expression tag |
| Met -5 | - | expression tag |
| Ala -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Ile -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 159 | Gly 177 | engineered mutation |
| Ala 177 | Asp 195 | engineered mutation |
| Arg 178 | Ala 196 | engineered mutation |
| Ser 179 | Met 197 | engineered mutation |
| Arg 184 | Pro 202 | engineered mutation |
| Gly 451 | - | expression tag |
| Gly 452 | - | expression tag |
| Ser 453 | - | expression tag |
| Gly 454 | - | expression tag |
| Gly 455 | - | expression tag |
| Ser 456 | - | expression tag |
| Gly 457 | - | expression tag |
| Gly 458 | - | expression tag |
| Ser 459 | - | expression tag |
| Met 460 | - | expression tag |
| Asp 461 | - | expression tag |
| Tyr 462 | - | expression tag |
| Lys 463 | - | expression tag |
| Asp 464 | - | expression tag |
| Asp 465 | - | expression tag |
| Asp 466 | - | expression tag |
| Asp 467 | - | expression tag |
| Lys 468 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 224867.3 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 6787.4 | |
| All* | Total formula weight | 231654.7 |
