5VL3
CD22 d1-d3 in complex with therapeutic Fab Epratuzumab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (Q, R, S, T) | B-cell receptor CD22 | polymer | 323 | 36802.4 | 4 | UniProt (P20273) Pfam (PF08205) Pfam (PF13927) | Homo sapiens (Human) | B-lymphocyte cell adhesion molecule,BL-CAM,Sialic acid-binding Ig-like lectin 2,Siglec-2,T-cell surface antigen Leu-14 |
| 2 | E, F, G, H (A, C, E, H) | Epratuzumab Fab Heavy Chain | polymer | 222 | 24028.7 | 4 | UniProt (S6B291) Pfam (PF07686) Pfam (PF07654) | Mus musculus (Mouse) | |
| 3 | I, J, K, L (B, D, F, L) | Epratuzumab Fab Light Chain | polymer | 222 | 24341.0 | 4 | UniProt (Q8TCD0) Pfam (PF07686) Pfam (PF07654) | Mus musculus (Mouse) | |
| 4 | M, O, Q, S (G, J, M, O) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 5 | N (I) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 6 | P, T (K, P) | alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 748.7 | 2 | In PDB GlyTouCan (G81315DD) | |||
| 7 | R (N) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 1 | In PDB GlyTouCan (G15407YE) | |||
| 8 | U (S) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) |
Sequence modifications
Q, R, S, T: 22 - 330 (UniProt: P20273)
A, C, E, H: -2 - 108 (PDB: 5VL3)
A, C, E, H: 109 - 217 (UniProt: S6B291)
B, D, F, L: -2 - 100 (PDB: 5VL3)
B, D, F, L: 101 - 215 (UniProt: Q8TCD0)
| PDB | External Database | Details |
|---|---|---|
| Thr 18 | - | expression tag |
| Gly 19 | - | expression tag |
| Asp 20 | - | expression tag |
| Ser 21 | - | expression tag |
| Gln 67 | Asn 67 | engineered mutation |
| Gln 112 | Asn 112 | engineered mutation |
| Gln 135 | Asn 135 | engineered mutation |
| Gln 164 | Asn 164 | engineered mutation |
| Gly 331 | - | expression tag |
| Gly 332 | - | expression tag |
| Thr 333 | - | expression tag |
| Lys 334 | - | expression tag |
| His 335 | - | expression tag |
| His 336 | - | expression tag |
| His 337 | - | expression tag |
| His 338 | - | expression tag |
| His 339 | - | expression tag |
| His 340 | - | expression tag |
A, C, E, H: 109 - 217 (UniProt: S6B291)
B, D, F, L: -2 - 100 (PDB: 5VL3)
B, D, F, L: 101 - 215 (UniProt: Q8TCD0)
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 340688.3 | |
| Branched | Number of molecules | 8 |
| Total formula weight | 4692.3 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 92.1 | |
| All* | Total formula weight | 345472.7 |
