5VJ1

Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with coenzyme A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, I	MdcA	polymer	554	61501.2	2	UniProt (Q9I6T0) Pfam (PF16957) In PDB	Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)	Malonate decarboxylase alpha subunit
2	C, K	MdcC	polymer	99	10693.0	2	UniProt (Q4K4F7) Pfam (PF06857) In PDB	Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)	Malonate decarboxylase acyl carrier protein, Malonate decarboxylase subunit delta
3	D, L	MdcD	polymer	287	30371.3	2	UniProt (A0A071KS24) Pfam (PF01039) UniProt (by SIFTS) (Q9I6S7) In PDB	Pseudomonas aeruginosa	Biotin-independent malonate decarboxylase subunit beta, Malonate decarboxylase subunit beta,Malonyl-S-ACP:biotin-protein carboxyltransferase MADC,Methylmalonyl-CoA carboxyltransferase 12S subunit
4	E, M	MdcE	polymer	284	30351.6	2	UniProt (A0A0C6EV56) Pfam (PF06833) UniProt (by SIFTS) (Q9I6S6) In PDB	Pseudomonas aeruginosa	Biotin-independent malonate decarboxylase subunit gamma, Malonate decarboxylase,gamma subunit,Malonyl-S-ACP:biotin-protein carboxyltransferase MADD
5	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
6	D, L	COENZYME A	non-polymer		767.5	2	Chemie (COA)

Sequence modifications

E, M: 1 - 268 (UniProt: A0A0C6EV56)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	265834.3
Non-Polymers*	Number of molecules	3
	Total formula weight	1570.5
All*	Total formula weight	267404.8

*Water molecules are not included.