5VJ1
Crystal structure of a Pseudomonas malonate decarboxylase hetero-tetramer in complex with coenzyme A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.740, 163.560, 100.440 |
Unit cell angles | 90.00, 94.01, 90.00 |
Refinement procedure
Resolution | 47.901 - 2.995 |
R-factor | 0.1762 |
Rwork | 0.173 |
R-free | 0.23320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.511 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.180 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.100 | 0.679 |
Number of reflections | 63210 | |
<I/σ(I)> | 12 | 1.7 |
Completeness [%] | 98.8 | 97.9 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 20% (w/v) PEG3350 and 8% Tacsimate |