5VEY

Solution NMR structure of histone H2A-H2B mono-ubiquitylated at H2A Lys15 in complex with RNF169 (653-708)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Histone H2B type 1-J,Histone H2A type 1-B/E	polymer	194	21658.9	1	UniProt (P06899) UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r,Histone H2A.2,Histone H2A/a,Histone H2A/m
2	B (B)	Polyubiquitin-B	polymer	76	8576.8	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
3	C (C)	E3 ubiquitin-protein ligase RNF169	polymer	59	7016.9	1	UniProt (Q8NCN4)	Homo sapiens (Human)	RING finger protein 169,RING-type E3 ubiquitin transferase RNF169

Sequence modifications

A: 9 - 99 (UniProt: P06899)

PDB	External Database	Details
Met 1	-	initiating methionine
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Met 8	-	expression tag
Ser 100	-	linker

A: 101 - 194 (UniProt: P04908)

PDB	External Database	Details
Ser 102	Lys 14	linker

C: 653 - 708 (UniProt: Q8NCN4)

PDB	External Database	Details
Gly 650	-	expression tag
His 651	-	expression tag
Met 652	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	37252.7
All*	Total formula weight	37252.7

*Water molecules are not included.