5VEY

Solution NMR structure of histone H2A-H2B mono-ubiquitylated at H2A Lys15 in complex with RNF169 (653-708)

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE III		700

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D TROSY 1H-15N HSQC
10	1	10	2D TROSY 1H-15N HSQC
100	2	23	3D HN(CA)CO
101	2	23	3D HBHA(CO)NH
102	2	23	3D CCCH-TOCSY
103	2	23	3D 1H-15N NOESY
104	2	23	3D 1H-13C NOESY-HSQC
105	2	23	13C-edited/filtered NOESY
106	2	24	3D CBCA(CO)NH
107	2	24	3D HNCACB
108	2	24	3D HNCO
109	2	24	3D HN(CA)CO
11	1	11	2D TROSY 1H-15N HSQC
110	2	24	3D HBHA(CO)NH
111	2	24	3D CCCH-TOCSY
112	2	24	3D 1H-15N NOESY
113	2	24	3D 1H-13C NOESY-HSQC
114	2	24	13C-edited/filtered NOESY
115	2	23	3D HCCCONH
116	2	24	3D HCCCONH
117	2	29	3D CBCA(CO)NH
118	2	29	3D HNCACB
119	2	29	3D HNCO
12	1	12	2D TROSY 1H-15N HSQC
120	2	29	3D HN(CA)CO
121	2	29	3D HBHA(CO)NH
122	2	29	3D HCCCONH
123	2	29	3D CCCH-TOCSY
124	2	29	3D 1H-15N NOESY
125	2	29	3D 1H-13C NOESY-HSQC
126	2	29	13C-edited/filtered NOESY
127	2	30	3D CBCA(CO)NH
128	2	30	3D HNCACB
129	2	30	3D HNCO
13	1	13	2D TROSY 1H-15N HSQC
130	2	30	3D HN(CA)CO
131	2	30	3D HBHA(CO)NH
132	2	30	3D HCCCONH
133	2	30	3D CCCH-TOCSY
134	2	30	3D 1H-15N NOESY
135	2	30	3D 1H-13C NOESY-HSQC
136	2	30	13C-edited/filtered NOESY
137	2	25	3D CBCA(CO)NH
138	2	25	3D HNCACB
139	2	25	3D HNCO
14	1	14	2D TROSY 1H-15N HSQC
140	2	25	3D HN(CA)CO
141	2	25	3D HBHA(CO)NH
142	2	25	3D HCCCONH
143	2	25	3D CCCH-TOCSY
144	2	25	3D 1H-15N NOESY
145	2	25	3D 1H-13C NOESY-HSQC
146	2	27	3D CBCA(CO)NH
147	2	27	3D HNCACB
148	2	27	3D HNCO
149	2	27	3D HN(CA)CO
15	1	15	2D TROSY 1H-15N HSQC
150	2	27	3D HBHA(CO)NH
151	2	27	3D HCCCONH
152	2	27	3D CCCH-TOCSY
153	2	28	3D CBCA(CO)NH
154	2	28	3D HNCACB
155	2	28	3D HNCO
156	2	28	3D HN(CA)CO
157	2	28	3D HBHA(CO)NH
158	2	28	3D HCCCONH
159	2	28	3D CCCH-TOCSY
16	1	16	2D TROSY 1H-15N HSQC
160	2	28	3D 1H-15N NOESY
161	1	18	3D 1H-13C NOESY-HSQC
162	1	16	3D 1H-15N NOESY
17	1	17	2D TROSY 1H-15N HSQC
18	1	18	2D TROSY 1H-15N HSQC
19	1	19	2D TROSY 1H-15N HSQC
2	1	2	2D TROSY 1H-15N HSQC
20	1	20	2D TROSY 1H-15N HSQC
21	1	21	2D TROSY 1H-15N HSQC
22	1	22	2D TROSY 1H-15N HSQC
23	2	23	2D 1H-15N HSQC
24	2	24	2D 1H-15N HSQC
25	2	25	2D 1H-15N HSQC
26	2	26	2D 1H-15N HSQC
27	2	27	2D 1H-15N HSQC
28	2	28	2D 1H-15N HSQC
29	2	29	2D 1H-15N HSQC
3	1	3	2D TROSY 1H-15N HSQC
30	2	30	2D 1H-15N HSQC
31	1	31	2D TROSY 1H-15N HSQC
32	1	32	2D TROSY 1H-15N HSQC
33	1	33	2D TROSY 1H-15N HSQC
34	1	34	2D TROSY 1H-15N HSQC
35	1	35	2D TROSY 1H-15N HSQC
36	1	36	2D TROSY 1H-15N HSQC
37	1	37	2D TROSY 1H-15N HSQC
38	1	38	2D TROSY 1H-15N HSQC
39	1	39	2D TROSY 1H-15N HSQC
4	1	4	22D TROSY 1H-15N HSQC
40	1	40	2D TROSY 1H-15N HSQC
41	1	41	2D TROSY 1H-15N HSQC
42	1	1	2D 1H-13C HMQC
43	1	1	2D 1H-13C HSQC
44	1	1	3D TROSY HNCO
45	1	1	3D TROSY HNCA
46	1	1	3D TROSY HN(CO)CA
47	1	1	3D TROSY HNCACB
48	1	1	3D TROSY HN(COCA)CB
49	1	3	3D HBHA(CO)NH
5	1	5	2D TROSY 1H-15N HSQC
50	1	3	3D HCCCONH
51	1	3	3D CCCH-TOCSY
52	1	3	3D 1H-15N NOESY
53	1	3	3D 1H-13C NOESY-HSQC
54	1	4	3D HBHA(CO)NH
55	1	4	3D HCCCONH
56	1	4	3D CCCH-TOCSY
57	1	4	3D 1H-15N NOESY
58	1	4	3D 1H-13C NOESY-HSQC
59	1	5	3D 1H-15N NOESY
6	1	6	2D TROSY 1H-15N HSQC
60	1	5	3D 1H-13C NOESY-HSQC
61	1	7	3D TROSY HNCO
62	1	7	3D TROSY HNCA
63	1	7	3D TROSY HN(CO)CA
64	1	7	3D TROSY HNCACB
65	1	7	3D TROSY HN(COCA)CB
66	1	8	3D HBHA(CO)NH
67	1	8	3D HCCCONH
68	1	8	3D CCCH-TOCSY
69	1	9	3D HBHA(CO)NH
7	1	7	2D TROSY 1H-15N HSQC
70	1	9	3D HCCCONH
71	1	9	3D CCCH-TOCSY
72	1	9	3D 1H-15N NOESY
73	1	9	3D 1H-13C NOESY-HSQC
74	1	10	3D 1H-15N NOESY
75	1	8	3D 1H-13C NOESY-HSQC
76	1	8	13C-edited/filtered NOESY
77	1	9	13C-edited/filtered NOESY
78	1	8	15N-edited/filtered NOESY
79	1	9	15N-edited/filtered NOESY
8	1	8	2D TROSY 1H-15N HSQC
80	1	8	3D 1H-13C NOESY aromatic
81	1	9	3D 1H-13C NOESY aromatic
82	1	20	2D 1H-13C HMQC
83	1	21	2D 1H-13C HMQC
84	1	20	3D 1H-13C NOESY-HMQC
85	1	21	3D 1H-13C NOESY-HMQC
86	1	20	3D 1H-13C NOESY-HSQC
87	1	21	3D 1H-13C NOESY-HSQC
88	1	12	3D 1H-13C NOESY
89	1	13	3D 1H-15N NOESY
9	1	9	2D TROSY 1H-15N HSQC
90	1	14	3D 1H-15N NOESY
91	1	15	3D 1H-13C NOESY
92	1	16	3D 1H-13C NOESY
93	1	17	3D 1H-15N NOESY
94	1	18	3D 1H-15N NOESY
95	1	19	3D 1H-13C NOESY
96	1	22	3D 1H-13C NOESY-HMQC
97	2	23	3D CBCA(CO)NH
98	2	23	3D HNCACB
99	2	23	3D HNCO

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6	1	303
2	6	1	298

Conformers

Conformers Calculated Total Number	200
Conformers Submitted Total Number	20