5VBL

Structure of apelin receptor in complex with agonist peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	agonist peptide	polymer	17	2383.3	1		Homo sapiens
2	B	Apelin receptor,Rubredoxin,Apelin receptor Chimera	polymer	407	46443.6	1	UniProt (P35414) UniProt (P00268) Pfam (PF00001) Pfam (PF00301) In PDB	Homo sapiens (Human)	Angiotensin receptor-like 1,G-protein coupled receptor APJ,G-protein coupled receptor HG11,Rd
3	B	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	B	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	1	Chemie (OLC)
5		water	water		18.0	4	Chemie (HOH)

Sequence modifications

B: 7 - 229 (UniProt: P35414)

PDB	External Database	Details
Met -17	-	initiating methionine
Lys -16	-	expression tag
Thr -15	-	expression tag
Ile -14	-	expression tag
Ile -13	-	expression tag
Ala -12	-	expression tag
Leu -11	-	expression tag
Ser -10	-	expression tag
Tyr -9	-	expression tag
Ile -8	-	expression tag
Phe -7	-	expression tag
Cys -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Phe -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Tyr 0	-	expression tag
Lys 1	-	expression tag
Asp 2	-	expression tag
Asp 3	-	expression tag
Asp 4	-	expression tag
Asp 5	-	expression tag
Lys 6	-	expression tag
Ala 117	Val 117	engineered mutation
Asn 177	Thr 177	engineered mutation

B: 1001 - 1054 (UniProt: P00268)
B: 243 - 330 (UniProt: P35414)

PDB	External Database	Details
Lys 261	Trp 261	engineered mutation
Leu 325	Cys 325	engineered mutation
Met 326	Cys 326	engineered mutation
Leu 331	-	expression tag
Glu 332	-	expression tag
Val 333	-	expression tag
Leu 334	-	expression tag
Phe 335	-	expression tag
Gln 336	-	expression tag
Gly 337	-	expression tag
Pro 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48827.0
Non-Polymers*	Number of molecules	2
	Total formula weight	421.9
All*	Total formula weight	49248.9

*Water molecules are not included.