5VBL
Structure of apelin receptor in complex with agonist peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 150.580, 44.300, 79.450 |
| Unit cell angles | 90.00, 114.87, 90.00 |
Refinement procedure
| Resolution | 41.610 - 2.600 |
| R-factor | 0.24 |
| Rwork | 0.239 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ej4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.610 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.488 | |
| Number of reflections | 13686 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 91.7 | 88.7 |
| Redundancy | 3.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100mM MES pH 6.1, 26% PEG500 DME, 125mM MgCl2, 100mM NaCl, 500uM AMG3054 |
