5VBA

Structure of EspG1 chaperone from the type VII (ESX-1) secretion system determined with the assistance of N-terminal T4 lysozyme fusion

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lysozyme, ESX-1 secretion-associated protein EspG1 chimera	polymer	437	47999.5	2	UniProt (D9IEF7) UniProt (X7YCN8) Pfam (PF00959) UniProt (by SIFTS) (P00720)	Enterobacteria phage T4	EspG1
2	C, D, E, F, G... (A, B)	CHLORIDE ION	non-polymer		35.5	10	Chemie (CL)
3	M, N (A, B)	water	water		18.0	56	Chemie (HOH)

Sequence modifications

A, B: 1002 - 1162 (UniProt: D9IEF7)

PDB	External Database	Details
Met 982	-	expression tag
Gly 983	-	expression tag
Ser 984	-	expression tag
Ser 985	-	expression tag
His 986	-	expression tag
His 987	-	expression tag
His 988	-	expression tag
His 989	-	expression tag
His 990	-	expression tag
His 991	-	expression tag
Ser 992	-	expression tag
Ser 993	-	expression tag
Gly 994	-	expression tag
Glu 995	-	expression tag
Asn 996	-	expression tag
Leu 997	-	expression tag
Tyr 998	-	expression tag
Phe 999	-	expression tag
Gln 1000	-	expression tag
Gly 1001	-	expression tag
Thr 1054	Cys 54	engineered mutation
Ala 1097	Cys 97	engineered mutation
Ala 1162	Lys 162	engineered mutation

A, B: 16 - 271 (UniProt: X7YCN8)

PDB	External Database	Details
Met 16	Val 16	engineered mutation
Ala 114	Cys 114	engineered mutation
Ala 170	Cys 170	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95999.0
Non-Polymers*	Number of molecules	10
	Total formula weight	354.5
All*	Total formula weight	96353.5

*Water molecules are not included.