5VBA

Structure of EspG1 chaperone from the type VII (ESX-1) secretion system determined with the assistance of N-terminal T4 lysozyme fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-05
Detector	RAYONIX MX300-HS
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	64.140, 81.690, 160.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.540 - 2.270
R-factor	0.216
Rwork	0.214
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 4GBR & 4L4W
RMSD bond length	0.004
RMSD bond angle	0.657
Data reduction software	XDS (November 3, 2014)
Data scaling software	XSCALE (November 3, 2014 BUILT=20141118)
Phasing software	PHASER (2.5.7)
Refinement software	PHENIX (DEV_2722)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.536	59.536	2.330
High resolution limit [Å]	2.270	10.150	2.270
Rmerge	0.097	0.048	1.110
Rmeas		0.054	1.206
Number of reflections	39581	505	2874
<I/σ(I)>	11.07	26.26	1.74
Completeness [%]	99.8	97.1	100
Redundancy	6.341	5.279	6.541
CC(1/2)		0.996	0.583

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	294	0.1 M Bicine, pH 9.0, 1.0 M lithium chloride, 10% PEG6000