5VAW

Fusion of Maltose-binding Protein and PilA from Acinetobacter baumannii AB5075

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose-binding periplasmic protein,Type IV pilin PilA	polymer	508	54134.6	1	UniProt (D8A942) UniProt (A0A0D5YCX7) Pfam (PF01547) Pfam (PF00114) UniProt (by SIFTS) (P0AEX9)	Escherichia coli	Type IV pilin structural subunit
2	B (A)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	1	Chemie (MPD)
3	C (A)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
4	D (A)	GLUTAMIC ACID	non-polymer		147.1	1	Chemie (GLU)
5	E (A)	LYSINE	non-polymer		147.2	1	Chemie (LYS)
6	F (A)	SERINE	non-polymer		105.1	1	Chemie (SER)
7	G (A)	water	water		18.0	297	Chemie (HOH)

Sequence modifications

A: 1 - 366 (UniProt: D8A942)

PDB	External Database	Details
Met 0	-	initiating methionine
Ala 82	Asp 108	engineered mutation
Ala 83	Lys 109	engineered mutation
Ala 87	Asp 113	engineered mutation
Ala 88	Lys 114	engineered mutation
Ala 172	Glu 198	engineered mutation
Ala 173	Asn 199	engineered mutation
Ala 239	Lys 265	engineered mutation
Ala 362	Lys 388	engineered mutation
Ala 363	Asp 389	engineered mutation
Asn 367	-	linker

A: 368 - 1151 (UniProt: A0A0D5YCX7)

PDB	External Database	Details
Ala 369	Ile 27	engineered mutation
Ala 370	Pro 28	engineered mutation
Leu 1152	-	expression tag
Glu 1153	-	expression tag
His 1154	-	expression tag
His 1155	-	expression tag
His 1156	-	expression tag
His 1157	-	expression tag
His 1158	-	expression tag
His 1159	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	54134.6
Non-Polymers*	Number of molecules	5
	Total formula weight	540.6
All*	Total formula weight	54675.2

*Water molecules are not included.