5V8S

Flavo di-iron protein H90D mutant from Thermotoga maritima

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Flavoprotein	polymer	410	46771.7	2	UniProt (Q9WZL4) Pfam (PF19583) Pfam (PF00258)	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
2	C, H (A, B)	MU-OXO-DIIRON	non-polymer		127.7	2	Chemie (FEO)
3	D, G, I (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4	E, J (A, B)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
5	F, K (A, B)	(4R)-2-METHYLPENTANE-2,4-DIOL	non-polymer		118.2	2	Chemie (MRD)
6	L, M (A, B)	water	water		18.0	515	Chemie (HOH)

Sequence modifications

A, B: 1 - 398 (UniProt: Q9WZL4)

PDB	External Database	Details
Met -11	-	initiating methionine
Gly -10	-	expression tag
Ser -9	-	expression tag
Asp -8	-	expression tag
Lys -7	-	expression tag
Ile -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Asp 90	His 90	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	93543.4
Non-Polymers*	Number of molecules	9
	Total formula weight	716.2
All*	Total formula weight	94259.5

*Water molecules are not included.