5V8S
Flavo di-iron protein H90D mutant from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.616, 91.857, 85.479 |
| Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
| Resolution | 84.980 - 1.410 |
| R-factor | 0.1513 |
| Rwork | 0.151 |
| R-free | 0.17790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dil |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.016 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.980 | 1.490 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rpim | 0.030 | 0.345 |
| Number of reflections | 158720 | 23232 |
| <I/σ(I)> | 13.2 | 2 |
| Completeness [%] | 98.6 | 99 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 0.1 M SODIUM ACETATE, 45% MPD |
