5V52

Structure of TIGIT bound to nectin-2 (CD112)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	T-cell immunoreceptor with Ig and ITIM domains	polymer	107	11672.0	2	UniProt (Q495A1) Pfam (PF07686)	Homo sapiens (Human)	V-set and immunoglobulin domain-containing protein 9,V-set and transmembrane domain-containing protein 3
2	C, D (C, D)	Nectin-2	polymer	140	15316.2	2	UniProt (Q92692) Pfam (PF07686)	Homo sapiens (Human)	Herpes virus entry mediator B,HveB,Nectin cell adhesion molecule 2,Poliovirus receptor-related protein 2
3	E (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	F (B)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	G (C)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)

Sequence modifications

A, B: 22 - 128 (UniProt: Q495A1)

PDB	External Database	Details
Ser 69	Cys 69	engineered mutation

C, D: 32 - 158 (UniProt: Q92692)

PDB	External Database	Details
Leu 159	-	expression tag
Val 160	-	expression tag
Pro 161	-	expression tag
Arg 162	-	expression tag
Gly 163	-	expression tag
Ser 164	-	expression tag
Gly 165	-	expression tag
His 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	53976.5
Non-Polymers*	Number of molecules	3
	Total formula weight	409.4
All*	Total formula weight	54385.8

*Water molecules are not included.