5V09
Crystal structure of human exonuclease 1 Exo1 (D225A) in complex with 5' recessed-end DNA (rVII)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | Z | Exonuclease 1 | polymer | 358 | 40310.8 | 1 | UniProt (Q9UQ84) Pfam (PF00752) Pfam (PF00867) In PDB | Homo sapiens (Human) | hExo1, Exonuclease I, hExoI |
| 2 | A | DNA (5'-D(*CP*GP*CP*TP*AP*GP*TP*CP*GP*TP*CP*AP*T)-3') | polymer | 13 | 3942.6 | 1 | synthetic construct | ||
| 3 | B | DNA (5'-D(P*AP*CP*GP*AP*CP*TP*AP*GP*CP*G)-3') | polymer | 10 | 3054.0 | 1 | synthetic construct | ||
| 4 | Z, A, B | MANGANESE (II) ION | non-polymer | 54.9 | 5 | Chemie (MN) | |||
| 5 | Z | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | water | water | 18.0 | 99 | Chemie (HOH) |
Sequence modifications
Z: 1 - 352 (UniProt: Q9UQ84)
| PDB | External Database | Details |
|---|---|---|
| Ala 225 | Asp 225 | engineered mutation |
| Glu 353 | - | expression tag |
| Asn 354 | - | expression tag |
| Leu 355 | - | expression tag |
| Tyr 356 | - | expression tag |
| Phe 357 | - | expression tag |
| Gln 358 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 47307.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 297.7 | |
| All* | Total formula weight | 47605.0 |
