5V09
Crystal structure of human exonuclease 1 Exo1 (D225A) in complex with 5' recessed-end DNA (rVII)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.904, 73.904, 180.081 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.594 - 2.750 |
R-factor | 0.2211 |
Rwork | 0.218 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qea |
RMSD bond length | 0.007 |
RMSD bond angle | 0.752 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.052 | 0.688 |
Rmeas | 0.056 | 0.750 |
Number of reflections | 13545 | 642 |
<I/σ(I)> | 36.46 | 3.27 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 6.3 | 6.5 |
CC(1/2) | 0.858 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000 |