5UZ7

Volta phase plate cryo-electron microscopy structure of a calcitonin receptor-heterotrimeric Gs protein complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	380	44326.2	1	UniProt (P63092) Pfam (PF00503) In PDB	Homo sapiens (Human)	Adenylate cyclase-stimulating G alpha protein
2	B	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	351	38744.4	1	UniProt (P62873) Pfam (PF00400) In PDB	Homo sapiens (Human)	Transducin beta chain 1
3	G	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	68	7563.8	1	UniProt (P59768) Pfam (PF00631) In PDB	Homo sapiens (Human)	G gamma-I
4	N	NANOBODY 35	polymer	138	15140.7	1		Lama Glama
5	R	Calcitonin receptor	polymer	501	58469.6	1	UniProt (P30988) Pfam (PF02793) Pfam (PF00002) In PDB	Homo sapiens (Human)	CT-R

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

R: 25 - 474 (UniProt: P30988)

PDB	External Database	Details
Met -7	-	initiating methionine
Lys -6	-	expression tag
Thr -5	-	expression tag
Ile -4	-	expression tag
Ile -3	-	expression tag
Ala -2	-	expression tag
Leu -1	-	expression tag
Ser 0	-	expression tag
Tyr 1	-	expression tag
Ile 2	-	expression tag
Phe 3	-	expression tag
Cys 4	-	expression tag
Leu 5	-	expression tag
Val 6	-	expression tag
Phe 7	-	expression tag
Ala 8	-	expression tag
Asp 9	-	expression tag
Tyr 10	-	expression tag
Lys 11	-	expression tag
Asp 12	-	expression tag
Asp 13	-	expression tag
Asp 14	-	expression tag
Asp 15	-	expression tag
Leu 16	-	expression tag
Glu 17	-	expression tag
Val 18	-	expression tag
Leu 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Gly 22	-	expression tag
Pro 23	-	expression tag
Ala 24	-	expression tag
Leu 447	Pro 447	conflict
Pro 475	-	expression tag
Ala 476	-	expression tag
Gly 477	-	expression tag
Leu 478	-	expression tag
Glu 479	-	expression tag
Val 480	-	expression tag
Leu 481	-	expression tag
Phe 482	-	expression tag
Gln 483	-	expression tag
Gly 484	-	expression tag
Pro 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag
His 493	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	164244.6
All*	Total formula weight	164244.6

*Water molecules are not included.