5UX0

X-ray crystal structure of Marinitoga piezophila Argonaute in complex with 5' OH guide RNA and target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, D)	Argonaute protein	polymer	642	76146.2	2	UniProt (H2J4R4) Pfam (PF22136) Pfam (PF22362)	Marinitoga piezophila
2	B, E (B, E)	RNA (5'-R(*G*GP*UP*AP*CP*AP*AP*CP*CP*UP*AP*CP*UP*AP*CP*CP*UP*CP*AP*U)-3')	polymer	21	6594.0	2		synthetic construct
3	C, F (C, F)	DNA (5'-D(P*AP*TP*GP*AP*GP*GP*TP*AP*GP*TP*AP*GP*GP*TP*TP*GP*TP*AP*CP*C)-3')	polymer	21	6542.2	2		synthetic construct

Sequence modifications

A, D: 2 - 639 (UniProt: H2J4R4)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag
Ala 516	Asp 516	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	178564.9
All*	Total formula weight	178564.9

*Water molecules are not included.