5UWR

Crystal Structure of CDC7 NES Peptide (extended) in complex with CRM1-Ran-RanBP1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GTP-binding nuclear protein Ran	polymer	237	26758.6	1	UniProt (P62826) Pfam (PF00071) In PDB	Homo sapiens (Human)	Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein
2	B	Ran-specific GTPase-activating protein 1	polymer	143	16521.9	1	UniProt (P41920) Pfam (PF00638) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Chromosome stability protein 20, Perinuclear array-localized protein, Ran-binding protein 1, RANBP1
3	C	Exportin-1	polymer	1024	117458.8	1	UniProt (P30822) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) In PDB	Saccharomyces cerevisiae (Baker's yeast)	Chromosome region maintenance protein 1, Karyopherin-124
4	D	Cell division cycle 7-related protein kinase	polymer	27	3019.4	1	UniProt (O00311) In PDB	Homo sapiens (Human)	huCdc7
5	A	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	1	Chemie (GNP)
6	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
7	A, C	GLYCEROL	non-polymer		92.1	3	Chemie (GOL)
8	C	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
9		water	water		18.0	522	Chemie (HOH)

Sequence modifications

A: 1 - 216 (UniProt: P62826)

PDB	External Database	Details
Met -20	-	expression tag
Glu -19	-	expression tag
Thr -18	-	expression tag
Gly -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Ser -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Leu -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag

B: 62 - 201 (UniProt: P41920)

PDB	External Database	Details
Gly 59	-	expression tag
Gly 60	-	expression tag
Ser 61	-	expression tag

C: 1 - 376 (UniProt: P30822)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 414 - 1058 (UniProt: P30822)

PDB	External Database	Details
Asp 441	Val 441	conflict
Gly 537	Asp 537	conflict
Cys 539	Thr 539	conflict
Glu 540	Val 540	conflict
Gln 541	Lys 541	conflict
Cys 1022	Tyr 1022	conflict

D: 456 - 478 (UniProt: O00311)

PDB	External Database	Details
Gly 452	-	expression tag
Gly 453	-	expression tag
Ser 454	-	expression tag
Tyr 455	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	163758.6
Non-Polymers*	Number of molecules	6
	Total formula weight	858.2
All*	Total formula weight	164616.8

*Water molecules are not included.