5UWR

Crystal Structure of CDC7 NES Peptide (extended) in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.085, 106.085, 304.551
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.443 - 2.240
R-factor	0.1879
Rwork	0.187
R-free	0.22210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.003
RMSD bond angle	0.527
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.240	2.240
Rmerge	0.114
Rpim	0.033	0.503
Number of reflections	84118	4085
<I/σ(I)>	23.8	1.5
Completeness [%]	99.4	99
Redundancy	12.6	12
CC(1/2)		0.384

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	17% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 10 mM spermine-HCl