5USR

Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E, G (A, C, E, G)	Cysteine desulfurase, mitochondrial	polymer	426	47623.3	4	UniProt (Q9Y697) Pfam (PF00266)	Homo sapiens (Human)
2	B, D, F, H (B, D, F, H)	LYR motif-containing protein 4	polymer	91	10763.5	4	UniProt (Q9HD34) Pfam (PF05347)	Homo sapiens (Human)
3	I, J, K, L (I, J, K, L)	Acyl carrier protein	polymer	78	8645.5	4	UniProt (P0A6A8) Pfam (PF00550)	Escherichia coli (strain K12)	ACP,Cytosolic-activating factor,CAF,Fatty acid synthase acyl carrier protein
4	M, N, O, P (I, J, K, L)	S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate	non-polymer		540.7	4	Chemie (8Q1)

Sequence modifications

A, C, E, G: 56 - 457 (UniProt: Q9Y697)

PDB	External Database	Details
Met 32	-	initiating methionine
Gly 33	-	expression tag
Ser 34	-	expression tag
Ser 35	-	expression tag
His 36	-	expression tag
His 37	-	expression tag
His 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
Ser 42	-	expression tag
Ser 43	-	expression tag
Gly 44	-	expression tag
Leu 45	-	expression tag
Val 46	-	expression tag
Pro 47	-	expression tag
Arg 48	-	expression tag
Gly 49	-	expression tag
Ser 50	-	expression tag
His 51	-	expression tag
Met 52	-	expression tag
Leu 53	-	expression tag
Glu 54	-	expression tag
Met 55	-	expression tag

B, D, F, H: 1 - 91 (UniProt: Q9HD34)

PDB	External Database	Details
Ala 11	Ser 11	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	268129.0
Non-Polymers*	Number of molecules	4
	Total formula weight	2162.6
All*	Total formula weight	270291.6

*Water molecules are not included.