5USR

Crystal structure of human NFS1-ISD11 in complex with E. coli acyl-carrier protein at 3.09 angstroms

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0030170	molecular_function	pyridoxal phosphate binding
A	0031071	molecular_function	cysteine desulfurase activity
A	0044571	biological_process	[2Fe-2S] cluster assembly
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005739	cellular_component	mitochondrion
B	0005759	cellular_component	mitochondrial matrix
B	0016226	biological_process	iron-sulfur cluster assembly
B	0016604	cellular_component	nuclear body
B	0042803	molecular_function	protein homodimerization activity
B	0044571	biological_process	[2Fe-2S] cluster assembly
B	0044572	biological_process	[4Fe-4S] cluster assembly
B	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
B	1990221	cellular_component	L-cysteine desulfurase complex
B	1990229	cellular_component	iron-sulfur cluster assembly complex
C	0030170	molecular_function	pyridoxal phosphate binding
C	0031071	molecular_function	cysteine desulfurase activity
C	0044571	biological_process	[2Fe-2S] cluster assembly
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005739	cellular_component	mitochondrion
D	0005759	cellular_component	mitochondrial matrix
D	0016226	biological_process	iron-sulfur cluster assembly
D	0016604	cellular_component	nuclear body
D	0042803	molecular_function	protein homodimerization activity
D	0044571	biological_process	[2Fe-2S] cluster assembly
D	0044572	biological_process	[4Fe-4S] cluster assembly
D	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
D	1990221	cellular_component	L-cysteine desulfurase complex
D	1990229	cellular_component	iron-sulfur cluster assembly complex
E	0030170	molecular_function	pyridoxal phosphate binding
E	0031071	molecular_function	cysteine desulfurase activity
E	0044571	biological_process	[2Fe-2S] cluster assembly
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005739	cellular_component	mitochondrion
F	0005759	cellular_component	mitochondrial matrix
F	0016226	biological_process	iron-sulfur cluster assembly
F	0016604	cellular_component	nuclear body
F	0042803	molecular_function	protein homodimerization activity
F	0044571	biological_process	[2Fe-2S] cluster assembly
F	0044572	biological_process	[4Fe-4S] cluster assembly
F	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
F	1990221	cellular_component	L-cysteine desulfurase complex
F	1990229	cellular_component	iron-sulfur cluster assembly complex
G	0030170	molecular_function	pyridoxal phosphate binding
G	0031071	molecular_function	cysteine desulfurase activity
G	0044571	biological_process	[2Fe-2S] cluster assembly
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005739	cellular_component	mitochondrion
H	0005759	cellular_component	mitochondrial matrix
H	0016226	biological_process	iron-sulfur cluster assembly
H	0016604	cellular_component	nuclear body
H	0042803	molecular_function	protein homodimerization activity
H	0044571	biological_process	[2Fe-2S] cluster assembly
H	0044572	biological_process	[4Fe-4S] cluster assembly
H	0099128	cellular_component	mitochondrial [2Fe-2S] assembly complex
H	1990221	cellular_component	L-cysteine desulfurase complex
H	1990229	cellular_component	iron-sulfur cluster assembly complex
I	0000035	molecular_function	acyl binding
I	0000036	molecular_function	acyl carrier activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0006633	biological_process	fatty acid biosynthetic process
I	0008289	molecular_function	lipid binding
I	0008610	biological_process	lipid biosynthetic process
I	0009245	biological_process	lipid A biosynthetic process
I	0009410	biological_process	response to xenobiotic stimulus
I	0031177	molecular_function	phosphopantetheine binding
J	0000035	molecular_function	acyl binding
J	0000036	molecular_function	acyl carrier activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0006633	biological_process	fatty acid biosynthetic process
J	0008289	molecular_function	lipid binding
J	0008610	biological_process	lipid biosynthetic process
J	0009245	biological_process	lipid A biosynthetic process
J	0009410	biological_process	response to xenobiotic stimulus
J	0031177	molecular_function	phosphopantetheine binding
K	0000035	molecular_function	acyl binding
K	0000036	molecular_function	acyl carrier activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006633	biological_process	fatty acid biosynthetic process
K	0008289	molecular_function	lipid binding
K	0008610	biological_process	lipid biosynthetic process
K	0009245	biological_process	lipid A biosynthetic process
K	0009410	biological_process	response to xenobiotic stimulus
K	0031177	molecular_function	phosphopantetheine binding
L	0000035	molecular_function	acyl binding
L	0000036	molecular_function	acyl carrier activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0005829	cellular_component	cytosol
L	0006633	biological_process	fatty acid biosynthetic process
L	0008289	molecular_function	lipid binding
L	0008610	biological_process	lipid biosynthetic process
L	0009245	biological_process	lipid A biosynthetic process
L	0009410	biological_process	response to xenobiotic stimulus
L	0031177	molecular_function	phosphopantetheine binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for Di-peptide 8Q1 I 101 and SER I 37

Chain	Residue
F	ARG6
F	ALA59
F	ASP62
I	ASP36
I	LEU38
I	ASP39
I	THR40
I	VAL41
F	LEU10
F	MET16
F	ALA39
F	ASN43
F	LYS44
F	VAL46
F	ILE52
F	LEU55

---

site_id	AC2
Number of Residues	18
Details	binding site for Di-peptide 8Q1 J 101 and SER J 37

Chain	Residue
G	ARG72
H	ARG6
H	VAL9
H	LEU10
H	ALA39
H	PHE40
H	ASN43
H	LYS44
H	VAL46
H	ILE52
H	LEU55
H	LYS58
H	ASP62
J	ASP36
J	LEU38
J	ASP39
J	THR40
J	VAL41

---

site_id	AC3
Number of Residues	15
Details	binding site for Di-peptide 8Q1 K 101 and SER K 37

Chain	Residue
B	ARG6
B	LEU10
B	MET16
B	ILE36
B	ALA39
B	ASN43
B	LYS44
B	ILE52
B	LEU55
B	ALA59
K	ASP36
K	LEU38
K	ASP39
K	THR40
K	VAL41

---

site_id	AC4
Number of Residues	14
Details	binding site for Di-peptide 8Q1 L 101 and SER L 37

Chain	Residue
C	ARG72
D	ARG6
D	ARG35
D	ILE36
D	ALA39
D	ASN43
D	LYS44
D	VAL46
D	LEU55
L	ASP36
L	LEU38
L	ASP39
L	THR40
L	VAL41

---

Functional Information from PROSITE/UniProt

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. DLGADSLDTVELVMAL

Chain	Residue	Details
I	ASP32-LEU47

---

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDLMsiSGHKiygpk.GvGaI

Chain	Residue	Details
A	ILE249-ILE268

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: O-(pantetheine 4'-phosphoryl)serine => ECO:0000255|PROSITE-ProRule:PRU00258, ECO:0000269|PubMed:4882207

Chain	Residue	Details
I	SER37
J	SER37
K	SER37
L	SER37
F	ARG6
F	LYS44
H	ARG6
H	LYS44

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q8K215

Chain	Residue	Details
B	LYS47
D	LYS47
F	LYS47
H	LYS47

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:29097656, ECO:0000269|PubMed:31101807, ECO:0000269|PubMed:34824239, ECO:0007744|PDB:5WKP, ECO:0007744|PDB:5WLW, ECO:0007744|PDB:6NZU, ECO:0007744|PDB:6UXE, ECO:0007744|PDB:6W1D, ECO:0007744|PDB:6WI2, ECO:0007744|PDB:6WIH, ECO:0007744|PDB:7RTK

Chain	Residue	Details
A	THR128
E	GLN235
E	SER255
E	HIS257
G	THR128
G	GLN235
G	SER255
G	HIS257
A	GLN235
A	SER255
A	HIS257
C	THR128
C	GLN235
C	SER255
C	HIS257
E	THR128

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:29097656, ECO:0000269|PubMed:31101807, ECO:0007744|PDB:5WKP, ECO:0007744|PDB:5WLW, ECO:0007744|PDB:6NZU

Chain	Residue	Details
A	THR295
C	THR295
E	THR295
G	THR295

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:29097656, ECO:0007744|PDB:5WLW

Chain	Residue	Details
A	CYS381
C	CYS381
E	CYS381
G	CYS381

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269|PubMed:29097656, ECO:0000269|PubMed:31101807, ECO:0000269|PubMed:34824239, ECO:0007744|PDB:5WKP, ECO:0007744|PDB:5WLW, ECO:0007744|PDB:6NZU, ECO:0007744|PDB:6UXE, ECO:0007744|PDB:6W1D, ECO:0007744|PDB:6WI2, ECO:0007744|PDB:6WIH, ECO:0007744|PDB:7RTK

Chain	Residue	Details
A	LLP258
C	LLP258
E	LLP258
G	LLP258

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	MOD_RES: Cysteine persulfide => ECO:0000305|PubMed:18650437, ECO:0000305|PubMed:23593335

Chain	Residue	Details
A	CYS381
C	CYS381
E	CYS381
G	CYS381

---