5US4

Crystal structure of human KRAS G12D mutant in complex with GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GTPase KRas	polymer	189	21501.2	2	UniProt (P01116) Pfam (PF00071)	Homo sapiens (Human)	K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras
2	C, F (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
3	D, G (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
4	E, H (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
5	I, J (A, B)	water	water		18.0	239	Chemie (HOH)

Sequence modifications

A, B: 1 - 169 (UniProt: P01116)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Asp 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	43002.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1119.2
All*	Total formula weight	44121.5

*Water molecules are not included.