5UQW

Crystal structure of human KRAS G12V mutant in complex with GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	GTPase KRas	polymer	189	21485.2	2	UniProt (P01116) Pfam (PF00071)	Homo sapiens (Human)	K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras
2	C, E (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, F (A, B)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
4	G, H (A, B)	water	water		18.0	298	Chemie (HOH)

Sequence modifications

A, B: 1 - 169 (UniProt: P01116)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Val 12	Gly 12	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	42970.4
Non-Polymers*	Number of molecules	4
	Total formula weight	935.0
All*	Total formula weight	43905.4

*Water molecules are not included.